MS2-TRAP (MS2-tagged RNA affinity purification): Tagging RNA to identify associated miRNAs
نویسندگان
چکیده
منابع مشابه
Purification of functional RNA-protein complexes using MS2-MBP.
Biological machines composed of RNAs and proteins play essential roles in many biological processes. To better understand the mechanism and function of these machines, it is critical to isolate them in a highly purified and functional form. A method for isolating functional RNA-protein complexes assembled in vitro is described. The approach combines gel filtration and an affinity-chromatography...
متن کاملMolecular interactions in the RNA bacteriophage MS2.
The simple RNA bacteriophages of E.coli have long been used as ideal model systems in which the details of RNA replication, translation and the control of gene expression can be studied in molecular detail’.’. In particular, the translational repression of the phage replicase cistron by coat protein subunits has attracted a great deal of attention and this has become a paradigm for sequence-spe...
متن کاملIdentification of sRNA interacting with a transcript of interest using MS2-affinity purification coupled with RNA sequencing (MAPS) technology
RNA sequencing (RNAseq) technology recently allowed the identification of thousands of small RNAs (sRNAs) within the prokaryotic kingdom. However, drawing the comprehensive interaction map of a sRNA remains a challenging task. To address this problem, we recently developed a method called MAPS (MS2 affinity purification coupled with RNA sequencing) to characterize the full targetome of specific...
متن کاملCodon usage and secondary structure of MS2 phage RNA.
MS2 is an RNA bacteriophage (3569 bases). The secondary structure of the RNA has been determined, and is known to play an important role in regulating translation. Paired regions of the genome have a higher G+C content than unpaired regions. It has been suggested that this reflects selection for high G+C content to encourage pairing, but a re-analysis of the data together with computer simulati...
متن کاملReplication of Viral Rna. Iv. Properties of Rna Synthetase and Enzymatic Synthesis of Ms2 Phage Rna.
Evidence has been presented' for the accumulation of a double-stranded replicative form of MS2 RNA in vivo, following infection of Escherichia coli with MS2 phage, and for the identity of this material with the double-stranded RNA which is labeled in vitro with radioactive carbon2 on incubation of CL4-labeled ribonucleoside triphosphates with RNA synthetase.3 The present paper is concerned with...
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ژورنال
عنوان ژورنال: Methods
سال: 2012
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2012.07.004